引物:引物是PCR特异性反应的关键,PCR 产物的特异性取决于引物与模板DNA互补的程度。理论上,只要知道任何一段模板DNA序列, 就能按其设计互补的寡核苷酸链做引物,利用PCR就可将模板DNA在体外大量扩增。设计引物应遵循以下原则:
③引物碱基:G+C含量以40-60%为宜,G+C太少扩增效果不佳,G+C过多易出现非特异条带。ATGC*随机分布,避免5个以上的嘌呤或嘧啶核苷酸的成串排列。
引物量:每条引物的浓度0.1~1umol或10~100pmol,以*引物量产生所需要的结果为好,引物浓度偏高会引起错配和非特异性扩增,且可增加引物之间形成二聚体的机会。
小鼠解整合素样金属蛋白酶9(ADAM9)ELISA Kit
Mouse A Disintegrin And Metalloprotease 9,ADAM9 ELISA Kit
小鼠解脲脲原体抗体(UU-Ab)ELISA Kit
Mouse Ureaplasma urealyticum antibody,UU-Ab ELISA Kit
小鼠睫状神经营养因子(CNTF)ELISA Kit
Mouse Ciliary Neurotrophic Factor,CNTF ELISA Kit
小鼠结蛋白(Des)ELISA Kit
Mouse Desmin,Des ELISA Kit
小鼠窖蛋白Caveolin1(Cav-1)ELISA Kit
Mouse Caveolin-1,CAV1 ELISA kit
小鼠角化细胞生长因子(KGF/FGF-7)ELISA Kit
Mouse Keratinocyte Growth Factor,KGF/FGF-7 ELISA Kit
小鼠胶原酶I(Collagenase I)ELISA Kit
Mouse Collagenase I ELISA Kit
小鼠胶原吡啶交联(PYD)ELISA Kit
Mouse pyridinoline,PYD ELISA Kit
小鼠降钙素原(PCT)ELISA Kit
Mouse Procalcitonin,PCT ELISA Kit
小鼠碱性磷酸酶(ALP)ELISA Kit
Mouse alkaline phosphatase,ALP ELISA Kit
小鼠碱性成纤维细胞生长因子(bFGF)ELISA Kit
Mouse basic fibroblast growth factor,bFGF ELISA Kit
小鼠甲状旁腺激素(PTH)ELISA Kit
Mouse parathyroid hormone,PTH ELISA kit
小鼠极低密度脂蛋白受体(VLDLR)ELISA Kit
Mouse very low density lipoprotein receptor,VLDLR ELISA Kit
小鼠极低密度脂蛋白(VLDL)ELISA Kit
Mouse very low density lipoprotein,VLDL ELISA Kit
小鼠基质细胞衍生因子1α(SDF-1α/SDF1A)ELISA Kit
Mouse stromal cell derived factor 1α,SDF-1α/SDF1A ELISA kit
小鼠基质金属蛋白酶抑制因子4(TIMP-4)ELISA Kit
Mouse tissue inhibitors of metalloproteinase 4,TIMP-4 ELISA kit
小鼠基质金属蛋白酶抑制因子3(TIMP-3)ELISA Kit
Mouse tissue inhibitors of metalloproteinase 3,TIMP-3 ELISA kit
小鼠基质金属蛋白酶抑制因子2(TIMP-2)ELISA Kit
Mouse tissue inhibitors of metalloproteinase 2,TIMP-2 ELISA kit
小鼠基质金属蛋白酶抑制因子1(TIMP-1)ELISA Kit
Mouse tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit
小鼠基质金属蛋白酶9/明胶酶B(MMP-9/Gelatinase B)ELISA Kit
Mouse matrix metalloproteinase 9/Gelatinase B,MMP-9 ELISA Kit